Development of Efficient Protocols for Stable and Transient Gene Transformation for Wolffia Globosa Using Agrobacterium.
Identifieur interne : 000186 ( Main/Exploration ); précédent : 000185; suivant : 000187Development of Efficient Protocols for Stable and Transient Gene Transformation for Wolffia Globosa Using Agrobacterium.
Auteurs : P P M. Heenatigala [République populaire de Chine, Sri Lanka] ; Jingjing Yang [République populaire de Chine] ; Anthony Bishopp [Royaume-Uni] ; Zuoliang Sun [République populaire de Chine] ; Gaojie Li [République populaire de Chine] ; Sunjeet Kumar [République populaire de Chine] ; Shiqi Hu [République populaire de Chine] ; Zhigang Wu [République populaire de Chine] ; Wei Lin [République populaire de Chine] ; Lunguang Yao [République populaire de Chine] ; Pengfei Duan [République populaire de Chine] ; Hongwei Hou [République populaire de Chine]Source :
- Frontiers in chemistry [ 2296-2646 ] ; 2018.
Abstract
Members of the Wolffia genus are fascinating plants for many biologists as they are the smallest flowering plants on Earth and exhibit a reduced body plan that is of great interest to developmental biologists. There has also been recent interest in the use of these species for bioenergy or biorefining. Molecular and developmental studies have been limited in Wolffia species due to the high genome complexity and uncertainties regarding the stable genetic transformation. In this manuscript we present new protocols for both stable and transient genetic transformation for Wolffia globosa using Agrobacterium tumefaciens. For the transient transformation, we used Wolffia fronds whereas we used clusters for the stable transformation. As proof of concept we transformed two synthetic promoter constructs driving expression of the GUS marker gene, that have previously been used to monitor auxin and cytokinin output in a variety of species. Using these approaches we obtained a Transformation Efficiency (TE) of 0.14% for the stable transformation and 21.8% for the transient transformation. The efficiency of these two methods of transformation are sufficient to allow future studies to investigate gene function. This is the first report for successful stable transformation of W. globosa.
DOI: 10.3389/fchem.2018.00227
PubMed: 29977889
PubMed Central: PMC6022245
Affiliations:
- Royaume-Uni, République populaire de Chine, Sri Lanka
- Angleterre, Hubei, Nottinghamshire
- Nottingham, Wuhan
- Université de Nottingham
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Heenatigala</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Inland Aquatic Resources and Aquaculture Division, National Aquatic Resources Research and Development Agency, Colombo, Sri 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Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Lin, Wei" sort="Lin, Wei" uniqKey="Lin W" first="Wei" last="Lin">Wei Lin</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yao, Lunguang" sort="Yao, Lunguang" uniqKey="Yao L" first="Lunguang" last="Yao">Lunguang Yao</name><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project, College of Agricultural Engineering, Nanyang Normal University, Nanyang, China.</nlm:affiliation><country 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University, Nanyang</wicri:regionArea><wicri:noRegion>Nanyang</wicri:noRegion></affiliation></author><author><name sortKey="Hou, Hongwei" sort="Hou, Hongwei" uniqKey="Hou H" first="Hongwei" last="Hou">Hongwei Hou</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region 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P M. Heenatigala</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Inland Aquatic Resources and Aquaculture Division, National Aquatic Resources Research and Development Agency, Colombo, Sri Lanka.</nlm:affiliation><country xml:lang="fr">Sri Lanka</country><wicri:regionArea>Inland Aquatic Resources and Aquaculture Division, National Aquatic Resources Research and Development Agency, Colombo</wicri:regionArea><wicri:noRegion>Colombo</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Jingjing" sort="Yang, Jingjing" uniqKey="Yang J" first="Jingjing" last="Yang">Jingjing Yang</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Bishopp, Anthony" sort="Bishopp, Anthony" uniqKey="Bishopp A" first="Anthony" last="Bishopp">Anthony Bishopp</name><affiliation wicri:level="4"><nlm:affiliation>Centre for Plant Integrative Biology, University of Nottingham, Nottingham, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Centre for Plant Integrative Biology, University of Nottingham, Nottingham</wicri:regionArea><orgName type="university">Université de Nottingham</orgName><placeName><settlement type="city">Nottingham</settlement><region type="nation">Angleterre</region><region type="région" nuts="1">Nottinghamshire</region></placeName></affiliation></author><author><name sortKey="Sun, Zuoliang" sort="Sun, Zuoliang" uniqKey="Sun Z" first="Zuoliang" last="Sun">Zuoliang Sun</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Li, Gaojie" sort="Li, Gaojie" uniqKey="Li G" first="Gaojie" last="Li">Gaojie Li</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Kumar, Sunjeet" sort="Kumar, Sunjeet" uniqKey="Kumar S" first="Sunjeet" last="Kumar">Sunjeet Kumar</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Hu, Shiqi" sort="Hu, Shiqi" uniqKey="Hu S" first="Shiqi" last="Hu">Shiqi Hu</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wu, Zhigang" sort="Wu, Zhigang" uniqKey="Wu Z" first="Zhigang" last="Wu">Zhigang Wu</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Lin, Wei" sort="Lin, Wei" uniqKey="Lin W" first="Wei" last="Lin">Wei Lin</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yao, Lunguang" sort="Yao, Lunguang" uniqKey="Yao L" first="Lunguang" last="Yao">Lunguang Yao</name><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project, College of Agricultural Engineering, Nanyang Normal University, Nanyang, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project, College of Agricultural Engineering, Nanyang Normal University, Nanyang</wicri:regionArea><wicri:noRegion>Nanyang</wicri:noRegion></affiliation></author><author><name sortKey="Duan, Pengfei" sort="Duan, Pengfei" uniqKey="Duan P" first="Pengfei" last="Duan">Pengfei Duan</name><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project, College of Agricultural Engineering, Nanyang Normal University, Nanyang, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project, College of Agricultural Engineering, Nanyang Normal University, Nanyang</wicri:regionArea><wicri:noRegion>Nanyang</wicri:noRegion></affiliation></author><author><name sortKey="Hou, Hongwei" sort="Hou, Hongwei" uniqKey="Hou H" first="Hongwei" last="Hou">Hongwei Hou</name><affiliation wicri:level="3"><nlm:affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></analytic><series><title level="j">Frontiers in chemistry</title><idno type="ISSN">2296-2646</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Members of the <i>Wolffia</i> genus are fascinating plants for many biologists as they are the smallest flowering plants on Earth and exhibit a reduced body plan that is of great interest to developmental biologists. There has also been recent interest in the use of these species for bioenergy or biorefining. Molecular and developmental studies have been limited in <i>Wolffia</i> species due to the high genome complexity and uncertainties regarding the stable genetic transformation. In this manuscript we present new protocols for both stable and transient genetic transformation for <i>Wolffia globosa</i> using <i>Agrobacterium tumefaciens</i>. For the transient transformation, we used <i>Wolffia</i> fronds whereas we used clusters for the stable transformation. As proof of concept we transformed two synthetic promoter constructs driving expression of the GUS marker gene, that have previously been used to monitor auxin and cytokinin output in a variety of species. Using these approaches we obtained a Transformation Efficiency (TE) of 0.14% for the stable transformation and 21.8% for the transient transformation. The efficiency of these two methods of transformation are sufficient to allow future studies to investigate gene function. This is the first report for successful stable transformation of <i>W. globosa</i>.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29977889</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2296-2646</ISSN><JournalIssue CitedMedium="Print"><Volume>6</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Frontiers in chemistry</Title><ISOAbbreviation>Front Chem</ISOAbbreviation></Journal><ArticleTitle>Development of Efficient Protocols for Stable and Transient Gene Transformation for <i>Wolffia Globosa</i> Using <i>Agrobacterium</i>.</ArticleTitle><Pagination><MedlinePgn>227</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fchem.2018.00227</ELocationID><Abstract><AbstractText>Members of the <i>Wolffia</i> genus are fascinating plants for many biologists as they are the smallest flowering plants on Earth and exhibit a reduced body plan that is of great interest to developmental biologists. There has also been recent interest in the use of these species for bioenergy or biorefining. Molecular and developmental studies have been limited in <i>Wolffia</i> species due to the high genome complexity and uncertainties regarding the stable genetic transformation. In this manuscript we present new protocols for both stable and transient genetic transformation for <i>Wolffia globosa</i> using <i>Agrobacterium tumefaciens</i>. For the transient transformation, we used <i>Wolffia</i> fronds whereas we used clusters for the stable transformation. As proof of concept we transformed two synthetic promoter constructs driving expression of the GUS marker gene, that have previously been used to monitor auxin and cytokinin output in a variety of species. Using these approaches we obtained a Transformation Efficiency (TE) of 0.14% for the stable transformation and 21.8% for the transient transformation. The efficiency of these two methods of transformation are sufficient to allow future studies to investigate gene function. This is the first report for successful stable transformation of <i>W. globosa</i>.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Heenatigala</LastName><ForeName>P P M</ForeName><Initials>PPM</Initials><AffiliationInfo><Affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Inland Aquatic Resources and Aquaculture Division, National Aquatic Resources Research and Development Agency, Colombo, Sri Lanka.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Jingjing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bishopp</LastName><ForeName>Anthony</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centre for Plant Integrative Biology, University of Nottingham, Nottingham, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Zuoliang</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Gaojie</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Sunjeet</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Shiqi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Zhigang</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>Lunguang</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Collaborative Innovation Center of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project, College of Agricultural Engineering, Nanyang Normal University, Nanyang, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Duan</LastName><ForeName>Pengfei</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Collaborative Innovation Center of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project, College of Agricultural Engineering, Nanyang Normal University, Nanyang, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hou</LastName><ForeName>Hongwei</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Wuhan, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>06</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Chem</MedlineTA><NlmUniqueID>101627988</NlmUniqueID><ISSNLinking>2296-2646</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Wolffia globosa</Keyword><Keyword MajorTopicYN="N">auxin</Keyword><Keyword MajorTopicYN="N">cytokinin</Keyword><Keyword MajorTopicYN="N">duckweed</Keyword><Keyword MajorTopicYN="N">stable transformation</Keyword><Keyword MajorTopicYN="N">transient transformation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>01</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>05</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29977889</ArticleId><ArticleId 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Aug;36(8):1225-1236</Citation><ArticleIdList><ArticleId IdType="pubmed">28466187</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Royaume-Uni</li><li>République populaire de Chine</li><li>Sri Lanka</li></country><region><li>Angleterre</li><li>Hubei</li><li>Nottinghamshire</li></region><settlement><li>Nottingham</li><li>Wuhan</li></settlement><orgName><li>Université de Nottingham</li></orgName></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Heenatigala, P P M" sort="Heenatigala, P P M" uniqKey="Heenatigala P" first="P P M" last="Heenatigala">P P M. Heenatigala</name></region><name sortKey="Duan, Pengfei" sort="Duan, Pengfei" uniqKey="Duan P" first="Pengfei" last="Duan">Pengfei Duan</name><name sortKey="Hou, Hongwei" sort="Hou, Hongwei" uniqKey="Hou H" first="Hongwei" last="Hou">Hongwei Hou</name><name sortKey="Hu, Shiqi" sort="Hu, Shiqi" uniqKey="Hu S" first="Shiqi" last="Hu">Shiqi Hu</name><name sortKey="Kumar, Sunjeet" sort="Kumar, Sunjeet" uniqKey="Kumar S" first="Sunjeet" last="Kumar">Sunjeet Kumar</name><name sortKey="Li, Gaojie" sort="Li, Gaojie" uniqKey="Li G" first="Gaojie" last="Li">Gaojie Li</name><name sortKey="Lin, Wei" sort="Lin, Wei" uniqKey="Lin W" first="Wei" last="Lin">Wei Lin</name><name sortKey="Sun, Zuoliang" sort="Sun, Zuoliang" uniqKey="Sun Z" first="Zuoliang" last="Sun">Zuoliang Sun</name><name sortKey="Wu, Zhigang" sort="Wu, Zhigang" uniqKey="Wu Z" first="Zhigang" last="Wu">Zhigang Wu</name><name sortKey="Yang, Jingjing" sort="Yang, Jingjing" uniqKey="Yang J" first="Jingjing" last="Yang">Jingjing Yang</name><name sortKey="Yao, Lunguang" sort="Yao, Lunguang" uniqKey="Yao L" first="Lunguang" last="Yao">Lunguang Yao</name></country><country name="Sri Lanka"><noRegion><name sortKey="Heenatigala, P P M" sort="Heenatigala, P P M" uniqKey="Heenatigala P" first="P P M" last="Heenatigala">P P M. Heenatigala</name></noRegion></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Bishopp, Anthony" sort="Bishopp, Anthony" uniqKey="Bishopp A" first="Anthony" last="Bishopp">Anthony Bishopp</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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